HDGF expression in various lysates analyzed by western blot. Primary antibody incubation was performed for 1 hour on 25ug protein per lane with Anti-HDGF Antibody (A89522) at a dilution of 1:1000 and detected with chemiluminescence.
HDGF expression in human lung cancer tissue analyzed by immunohistochemistry. Tissue was paraffin-embedded, and antigen retrieval was achieved with 10 mM citrate buffer, pH 6.0, under high pressure. Staining was performed with Anti-HDGF Antibody (A89522) at a dilution of 1:50.
HDGF expression in human thyroid cancer tissue analyzed by immunohistochemistry. Tissue was paraffin-embedded, and antigen retrieval was achieved with 10 mM citrate buffer, pH 6.0, under high pressure. Staining was performed with Anti-HDGF Antibody (A89522) at a dilution of 1:50.
HDGF expression in human breast cancer tissue analyzed by immunohistochemistry. Tissue was paraffin-embedded, and antigen retrieval was achieved with 10 mM citrate buffer, pH 6.0, under high pressure. Staining was performed with Anti-HDGF Antibody (A89522) at a dilution of 1:50.
HDGF expression in Hela cells analyzed by immunofluorescence. Staining was performed with Anti-HDGF Antibody (A89522) at a dilution of 1:50. Nuclei were stained with DAPI (blue).