The antibody detects endogenous level of total HDAC4 / HDAC5 / HDAC9 protein.
Applications
WB, IHC, IF
Dilutions
Western blotting: 1:500~1:1000 Immunohistochemistry: 1:50~1:100 Immunofluorescence: 1:100~1:200
Reactivity
Human, Mouse
Immunogen
Peptide sequence around aa.244~248 / 257~261 / 218~222 (T-A-S-E-P) derived from Human HDAC4 / HDAC5 / HDAC9.
Host
Rabbit
Clonality
Polyclonal
Conjugate
Unconjugated
Purification
Antibodies were produced by immunizing rabbits with synthetic peptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific peptide.
Concentration
1 mg/ml
Predicted MW
140 124 111 kd
Formulation
Supplied at 1.0mg / mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage
Store at -20°C for long term preservation (recommended). Store at 4°C for short term use.
Immunohistochemical analysis of paraffin-embedded human lung carcinoma tissue using HDAC4/HDAC5/HDAC9 (Ab-246/259/220) Antibody #21517 (left) or the same antibody preincubated with blocking peptide (right).
Immunofluorescence staining of methanol-fixed Hela cells using HDAC4/HDAC5/HDAC9 (Ab-246/259/220) Antibody #21517.
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