HDAC1 enrichment at the p21 locus of 1µg MCF7 cell chromatin analyzed by ChIP. ChIP was performed using the Chromatrap® spin column kit (Protein G) and Anti-HDAC1 Antibody (A82481).
HDAC1 expression in NIH3T3 cell lysate analyzed by western blot. Cells were lysed in RIPA buffer and 35µg protein was run per lane. Primary antibody incubation was performed for 1 hour with Anti-HDAC1 Antibody (A82481) at 0.3µg/ml and detected by chemiluminescence.
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