Anti-GST Tag Antibody (A17322)

Western blot analysis of over-expressed GST protein using Anti-GST Tag Antibody (A17322) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 1s.

Western blot analysis of over-expressed GST protein using Anti-GST Tag Antibody (A17322) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 1s.

Immunoprecipitation analysis of 50µg extracts of GST Protein using 3µg of Anti-GST Tag Antibody (A17322). This Western blot was performed on the immunoprecipitate using Anti-GST Tag Antibody (A17322).

Immunoprecipitation analysis of 300µg extracts of GST-protein cells using 3µg of Anti-GST Tag Antibody (A17322). This Western blot was performed on the immunoprecipitate using Anti-GST Tag Antibody (A17322) at a dilution of 1:10000.