Anti-GSK3 beta (phospho Ser9) Antibody [ARC0069] (A308989)

Name: Anti-GSK3 beta (phospho Ser9) Antibody [ARC0069]
Brand: Antibodies.com
SKU: A308989
Price: 485.0 USD
Availability: InStock

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$485

Rabbit monoclonal [ARC0069] antibody to GSK3 beta (phospho Ser9) for WB and IHC.

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Shipping Information

Freight/Packing Charges: $40

Dispatched from St. Louis, MO.

Lead Time: 7-10 business days.

Specifications

Name

Anti-GSK3 beta (phospho Ser9) Antibody [ARC0069]

Description

Rabbit monoclonal [ARC0069] antibody to GSK3 beta (phospho Ser9).

Applications

WB, IHC

Dilutions

WB: 1:500-1:2,000, IHC: 1:50-1:200

Reactivity

Human, Mouse

Immunogen

A synthetic phosphorylated peptide around S9 of human GSK3 beta (P49841).

Sequence

TTSFA

Host

Rabbit

Clonality

Monoclonal

Clone ID

ARC0069

Isotype

IgG

Conjugate

Unconjugated

Purification

Affinity purification.

Concentration

Lot Specific

Molecular Weight

46 kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol, 0.05% BSA, and 0.02% Sodium Azide.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze/thaw cycles.

Synonyms

EC:2.7.11.1, Glycogen synthase kinase-3 beta, GSK3B, Serine/threonine-protein kinase GSK3B

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation DataValidation Data (2)

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Western Blot - Anti-GSK3 beta (phospho Ser9) Antibody [ARC0069] (A308989)

Western blot analysis of extracts of various cell lines, using Anti-GSK3 beta (phospho Ser9) Antibody [ARC0069] (A308989) at 1:1,000 dilution. HeLa and NIH/3T3 cells were treated by Calyculin A (100 nM) at 37°C for 30 minutes after serum-starvation overnight. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 1s.

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Immunohistochemistry - Anti-GSK3 beta (phospho Ser9) Antibody [ARC0069] (A308989)

Immunohistochemistry analysis of paraffin-embedded human colon carcinoma tissue using Anti-GSK3 beta (phospho Ser9) Antibody [ARC0069] (A308989) at a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

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