Anti-GSDMD Antibody [ARC61812] (A329423)

Name: Anti-GSDMD Antibody [ARC61812]
Brand: Antibodies.com
SKU: A329423
Price: 385.0 USD
Availability: InStock

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$385

Rabbit monoclonal [ARC61812] antibody to GSDMD for WB and ELISA.

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Shipping Information

Freight/Packing Charges: $40

Dispatched from St. Louis, MO.

Lead Time: 7-10 business days.

Specifications

Name

Anti-GSDMD Antibody [ARC61812]

Description

Rabbit monoclonal [ARC61812] antibody to GSDMD.

Applications

WB, ELISA

Positive Control

THP-1 treated by LPS and Nigericin, Recombination GSDMD-FL and N+C Protein.

Dilutions

WB: 1:2,000-1:12,000, ELISA: 1 µg/ml (starting concentration)

Reactivity

Human

Immunogen

A recombinant fusion protein corresponding to amino acids 276-484 of human GSDMD.

Sequence

GVPAEGAFTEDFQGLRAEVETISKELELLDRELCQLLLEGLEGVLRDQLALRALEEALEQGQSLGPVEPLDGPAGAVLECLVLSSGMLVPELAIPVVYLLGALTMLSETQHKLLAEALESQTLLGPLELVGSLLEQSAPWQERSTMSLPPGLLGNSWGEGAPAWVLLDECGLELGEDTPHVCWEPQAQGRMCALYASLALLSGLSQEPH

Host

Rabbit

Clonality

Monoclonal

Clone ID

ARC61812

Isotype

IgG

Conjugate

Unconjugated

Purification

Affinity purification.

Concentration

Lot Specific

Molecular Weight

23 kDa / 53 kDa

Predicted MW

53 kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.3, with 0.05% BSA, 50% Glycerol, and 0.05% ProClin 300.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze/thaw cycles.

Synonyms

DFNA5L, Gasdermin domain-containing protein 1, Gasdermin-D, GSDMDC1

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation DataValidation Data (2)

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Western Blot - Anti-GSDMD Antibody [ARC61812] (A329423)

GSDMD expression in THP-1 cell lysates analyzed by western blot. Cells were treated with LPS and Nigericin. Primary antibody incubation was performed for 1 hour on 25ug protein per lane with Anti-GSDMD Antibody [ARC61812] (A329423) at a dilution of 1:2000 and detected with chemiluminescence.

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Western Blot - Anti-GSDMD Antibody [ARC61812] (A329423)

GSDMD expression usingrecombinant GSDMD protein analyzed by western blot Primary antibody incubation was performed for 1 hour on 10ng protein per lane with Anti-GSDMD Antibody [ARC61812] (A329423) at a dilution of 1:2000 and detected with chemiluminescence.

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