Anti-GRP94 Antibody [ARC60312] (A329418)

Name: Anti-GRP94 Antibody [ARC60312]
Brand: Antibodies.com
SKU: A329418
Price: 485.0 USD
Availability: InStock

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$485

Rabbit monoclonal [ARC60312] antibody to GRP94 for WB, IHC-P and ELISA.

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Shipping Information

Freight/Packing Charges: $40

Dispatched from St. Louis, MO.

Lead Time: 7-10 business days.

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Specifications

Name

Anti-GRP94 Antibody [ARC60312]

Description

Rabbit monoclonal [ARC60312] antibody to GRP94.

Applications

WB, IHC-P, ELISA

Positive Control

HeLa, MCF7, NIH/3T3, PC-12.

Dilutions

WB: 1:1,000-1:5,000, IHC-P: 1:50-1:200, ELISA: 1 µg/ml (starting concentration)

Reactivity

Human, Mouse, Rat

Immunogen

A synthetic peptide corresponding to amino acids 616-715 of human GRP94.

Sequence

EPLLNWMKDKALKDKIEKAVVSQRLTESPCALVASQYGWSGNMERIMKAQAYQTGKDISTNYYASQKKTFEINPRHPLIRDMLRRIKEDEDDKTVLDLAV

Host

Rabbit

Clonality

Monoclonal

Clone ID

ARC60312

Isotype

IgG

Conjugate

Unconjugated

Purification

Affinity purification.

Concentration

Lot Specific

Molecular Weight

100 kDa

Predicted MW

92 kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.3, with 0.05% BSA, 50% Glycerol, and 0.05% ProClin 300.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze/thaw cycles.

Synonyms

94 kDa glucose-regulated protein, Endoplasmin, gp96 homolog, GRP-94, Heat shock protein 90 kDa beta member 1, Heat shock protein family C member 4, HSP90B1, HSPC4, TRA1, Tumor rejection antigen 1

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation DataValidation Data (8)

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Western Blot - Anti-GRP94 Antibody [ARC60312] (A329418)

GRP94 expression in lysates from wild type (WT) and HSP90B1 knockdown (KD) HeLa cells analyzed by western blot. Primary antibody incubation was performed for 1 hour on 25ug protein per lane with Anti-GRP94 Antibody [ARC60312] (A329418) at a dilution of 1:2000 and detected with chemiluminescence.

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Western Blot - Anti-GRP94 Antibody [ARC60312] (A329418)

GRP94 expression in various lysates analyzed by western blot. Primary antibody incubation was performed for 1 hour on 25ug protein per lane with Anti-GRP94 Antibody [ARC60312] (A329418) at a dilution of 1:2000 and detected with chemiluminescence.

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Immunohistochemistry - Anti-GRP94 Antibody [ARC60312] (A329418)

GRP94 expression in rat liver tissue analyzed by immunohistochemistry. Tissue was paraffin-embedded, and antigen retrieval was achieved with 10 mM citrate buffer, pH 6.0, under high pressure. Staining was performed with Anti-GRP94 Antibody [ARC60312] (A329418) at a dilution of 1:200.

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Immunohistochemistry - Anti-GRP94 Antibody [ARC60312] (A329418)

GRP94 expression in rat brain tissue analyzed by immunohistochemistry. Tissue was paraffin-embedded, and antigen retrieval was achieved with 10 mM citrate buffer, pH 6.0, under high pressure. Staining was performed with Anti-GRP94 Antibody [ARC60312] (A329418) at a dilution of 1:200.