Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol and 0.01% Thiomersal.
Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Synonyms
94 kDa glucose-regulated protein, Endoplasmin, gp96 homolog, GRP-94, Heat shock protein 90 kDa beta member 1, Heat shock protein family C member 4, HSP90B1, HSPC4, TRA1, Tumor rejection antigen 1
Western blot analysis of extracts of various cell lines, using Anti-GRP94 Antibody (A91465) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 30s.
Western blot analysis of extracts of various cell lines, using Anti-GRP94 Antibody (A91465) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 180s.
Immunohistochemistry analysis of paraffin-embedded rat spleen using Anti-GRP94 Antibody (A91465) at a dilution of 1:50 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
Immunohistochemistry analysis of paraffin-embedded human liver cancer using Anti-GRP94 Antibody (A91465) at a dilution of 1:50 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
Immunohistochemistry analysis of paraffin-embedded mouse brain using Anti-GRP94 Antibody (A91465) at a dilution of 1:50 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
Immunofluorescence analysis of NIH-3T3 cells using Anti-GRP94 Antibody (A91465) at a dilution of 1:100 (40x lens). DAPI was used to stain the cell nuclei (blue).
![Immunofluorescence - Anti-GRP94 Antibody [SPM249] - BSA and Azide free (A253390) - Antibodies.com](https://cdn.antibodies.com/image/catalog/253/A253390_1.jpg?profile=product_alternative)
![Immunohistochemistry - Anti-GRP94 Antibody [9G10.F8.2] (A250209) - Antibodies.com](https://cdn.antibodies.com/image/catalog/250/A250209_1.jpg?profile=product_alternative)
![Immunohistochemistry - Anti-GRP94 Antibody [9G10.F8.2] - BSA and Azide free (A253389) - Antibodies.com](https://cdn.antibodies.com/image/catalog/253/A253389_1.jpg?profile=product_alternative)
![Immunofluorescence - Anti-GRP94 Antibody [SPM249] (A250210) - Antibodies.com](https://cdn.antibodies.com/image/catalog/250/A250210_1.jpg?profile=product_alternative)
![SDS-PAGE - Anti-GRP94 Antibody [HSP90B1/3168R] - BSA and Azide free (A253388) - Antibodies.com](https://cdn.antibodies.com/image/catalog/253/A253388_1.jpg?profile=product_alternative)
![Western Blot - Anti-GRP94 Antibody [9G10] (A305089) - Antibodies.com](https://cdn.antibodies.com/image/catalog/305/A305089_1.png?profile=product_alternative)
![SDS-PAGE - Anti-GRP94 Antibody [HSP90B1/3168R] (A250208) - Antibodies.com](https://cdn.antibodies.com/image/catalog/250/A250208_1.jpg?profile=product_alternative)
