Synthetic peptide corresponding to the internal region of human GRP94.
Sequence
C-KEGVKFDESEKTKE
Host
Goat
Clonality
Polyclonal
Isotype
IgG
Conjugate
Unconjugated
Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Concentration
500 µg/ml
Molecular Weight
100 kDa
Predicted MW
92.5kDa
Product Form
Liquid
Formulation
Supplied in Tris Buffered Saline, pH 7.3, with 0.5% BSA and 0.02% Sodium Azide.
Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze/thaw cycles.
Synonyms
94 kDa glucose-regulated protein, Endoplasmin, gp96 homolog, GRP-94, Heat shock protein 90 kDa beta member 1, Heat shock protein family C member 4, HSP90B1, HSPC4, TRA1, Tumor rejection antigen 1
GRP94 expression in U2OS (A), HEK293 (B), HeLa (C), and A549 (D) cell lysates analyzed by western blot. Cells were lysed in RIPA buffer and 35µg protein was run per lane. Primary antibody incubation was performed with Anti-GRP94 Antibody (A84222) at 0.1µg/ml (A-C) or 0.3µg/ml (D) and detected by chemiluminescence.
GRP94 expression in NIH3T3 cell lysate analyzed by western blot. Cells were lysed in RIPA buffer and 35µg protein was run per lane. Primary antibody incubation was performed with Anti-GRP94 Antibody (A84222) at 0.1µg/ml and detected by chemiluminescence.
GRP94 expression in A431 cells analyzed by immunofluorescence. Cells were permeabilized with 0.15% Triton. Staining was performed with Anti-GRP94 Antibody (A84222) at 10µg/ml for 1 hour and Alexa Fluor 488 secondary antibody at 2µg/ml. Cytoplasmic and ER staining shown and nuclei were stained with DAPI (blue). Negative control: Goat IgG Isotype Control at 10µg/ml followed by Alexa Fluor 488 secondary antibody at 2µg/ml.
GRP94 expression in HeLa cells analyzed by immunofluorescence. Cells were permeabilized with 0.15% Triton. Staining was performed with Anti-GRP94 Antibody (A84222) at 10µg/ml for 1 hour and Alexa Fluor 488 secondary antibody at 2µg/ml. Cytoplasmic and ER staining shown and nuclei were stained with DAPI (blue). Negative control: Goat IgG Isotype Control at 10µg/ml followed by Alexa Fluor 488 secondary antibody at 2µg/ml.
GRP94 expression in A431 cells (blue line) analyzed by flow cytometry. Cells were fixed in PFA and permeabilized with 0.5% Triton. Staining was performed with Anti-GRP94 Antibody (A84222) at 10µg/ml for 1 hour and Alexa Fluor 488 secondary antibody at 1µg/ml. Negative Control: Goat IgG Isotype Control (black line) followed by Alexa Fluor 488 secondary antibody.
GRP94 expression in Human Liver analyzed by immunohistochemistry. Tissue was paraffin-embedded, and antigen retrieval was achieved by steaming in citrate buffer, pH 6. Staining was performed with Anti-GRP94 Antibody (A84222) at 3.8µg/ml and revealed with alkaline phosphatase (AP).
GRP94 expression in Human Uterus analyzed by immunohistochemistry. Tissue was paraffin-embedded, and antigen retrieval was achieved by steaming in citrate buffer, pH 6. Staining was performed with Anti-GRP94 Antibody (A84222) at 5µg/ml and revealed with alkaline phosphatase (AP).
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![Immunohistochemistry - Anti-GRP94 Antibody [9G10.F8.2] (A250209) - Antibodies.com](https://cdn.antibodies.com/image/catalog/250/A250209_1.jpg?profile=product_alternative)
![Immunohistochemistry - Anti-GRP94 Antibody [9G10.F8.2] - BSA and Azide free (A253389) - Antibodies.com](https://cdn.antibodies.com/image/catalog/253/A253389_1.jpg?profile=product_alternative)
![Immunofluorescence - Anti-GRP94 Antibody [SPM249] (A250210) - Antibodies.com](https://cdn.antibodies.com/image/catalog/250/A250210_1.jpg?profile=product_alternative)
![SDS-PAGE - Anti-GRP94 Antibody [HSP90B1/3168R] - BSA and Azide free (A253388) - Antibodies.com](https://cdn.antibodies.com/image/catalog/253/A253388_1.jpg?profile=product_alternative)
![Western Blot - Anti-GRP94 Antibody [9G10] (A305089) - Antibodies.com](https://cdn.antibodies.com/image/catalog/305/A305089_1.png?profile=product_alternative)
![SDS-PAGE - Anti-GRP94 Antibody [HSP90B1/3168R] (A250208) - Antibodies.com](https://cdn.antibodies.com/image/catalog/250/A250208_1.jpg?profile=product_alternative)
