Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol and 0.02% Sodium Azide.
Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Synonyms
94 kDa glucose-regulated protein, Endoplasmin, gp96 homolog, GRP-94, Heat shock protein 90 kDa beta member 1, Heat shock protein family C member 4, HSP90B1, HSPC4, TRA1, Tumor rejection antigen 1
Western blot analysis of extracts of various cell lines, using Anti-GRP94 Antibody (A305806) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Enhanced Kit (RM00021). Exposure time: 90s.
Immunofluorescence analysis of HeLa cells using Anti-GRP94 Antibody (A305806) at a dilution of 1:100 (40x lens). DAPI was used to stain the cell nuclei (blue).
Immunofluorescence analysis of NIH/3T3 cells using Anti-GRP94 Antibody (A305806) at a dilution of 1:100 (40x lens). DAPI was used to stain the cell nuclei (blue).
Immunofluorescence analysis of PC-12 cells using Anti-GRP94 Antibody (A305806) at a dilution of 1:100 (40x lens). DAPI was used to stain the cell nuclei (blue).
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