Rabbit polyclonal antibody to GRB2 (Phospho-Ser159)
Specificity
The antibody detects endogenous levels of GRB2 only when phosphorylated at serine 159.
Applications
WB
Dilutions
Western blotting: 1:500~1:1000
Reactivity
Human
Immunogen
Peptide sequence around phosphorylation site of Serine 159(K-S-S(p)-A-P) derived from Human GRB2.
Host
Rabbit
Clonality
Polyclonal
Conjugate
Unconjugated
Purification
Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
Concentration
1 mg/ml
Formulation
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage
Store at -20˚C / 1 year
Synonyms
Adapter protein GRB2, ASH, Growth factor receptor-bound protein 2, Protein Ash, SH2/SH3 adapter GRB2
Western blot analysis of extracts from HT-29 cells treated with serum using GRB2 (Phospho-Ser159) Antibody #11771.The lane on the right is treated with the antigen-specific peptide.
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