GRB2 expression in Human Thymus (A) and MOLT4 (B) lysates analyzed by western blot. Cells were lysed in RIPA buffer and 35µg protein was run per lane. Primary antibody incubation was performed for 1 hour with Anti-GRB2 Antibody (A82905) at 0.05µg/ml and detected by chemiluminescence.
GRB2 expression in Human (A) and Pig (B) Spleen cell lysates analyzed by western blot. Cells were lysed in RIPA buffer and 35µg protein was run per lane. Primary antibody incubation was performed for 1 hour with Anti-GRB2 Antibody (A82905) at 0.03µg/ml and detected by chemiluminescence.
GRB2 expression in Mouse (A+C) and Rat (B+D) Brain (A+B) and Spleen (C+D) lysates analyzed by western blot. Cells were lysed in RIPA buffer and 35µg protein was run per lane. Primary antibody incubation was performed for 1 hour with Anti-GRB2 Antibody (A82905) at 0.05µg/ml and detected by chemiluminescence.
GRB2 expression in Human Lymph Node analyzed by immunohistochemistry. Tissue was paraffin-embedded, and antigen retrieval was achieved by microwaving in citrate buffer, pH 6. Staining was performed with Anti-GRB2 Antibody (A82905) at 1µg/ml and revealed with horseradish peroxidase (HRP).
Negative control for GRB2 expression in Human Lymph Node analyzed by immunohistochemistry. Tissue was paraffin-embedded, and staining procedure was performed in the absence of primary antibody.
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