Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol, 0.05% BSA, and 0.02% Sodium Azide.
Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Synonyms
Golgi peripheral membrane protein p65, Golgi phosphoprotein 5, Golgi reassembly-stacking protein 1, Golgi reassembly-stacking protein of 65 kDa, GOLPH5, GORASP1
Western Blot - Anti-GRASP65 Antibody [ARC1911] (A308675)
Western blot analysis of extracts of various cell lines, using Anti-GRASP65 Antibody [ARC1911] (A308675) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 10s.
Western Blot - Anti-GRASP65 Antibody [ARC1911] (A308675)
Western blot analysis of extracts of various cell lines, using Anti-GRASP65 Antibody [ARC1911] (A308675) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 3min.
Immunofluorescence analysis of U-2 OS cells using Anti-GRASP65 Antibody [ARC1911] (A308675) at a dilution of 1:100 (40x lens). DAPI was used to stain the cell nuclei (blue).
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