Anti-Granzyme B Antibody [CLB-GB11] (FITC) (A254409)

Name: Anti-Granzyme B Antibody [CLB-GB11] (FITC)
Brand: Antibodies.com
SKU: A254409
Price: 880.0 USD
Availability: InStock

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$880

Mouse monoclonal [CLB-GB11] antibody to Granzyme B (FITC) for Flow Cytometry.

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Alternative Formats and ConjugatesAlternative Formats

Unconjugated

Shipping Information

Freight/Packing Charges: $40

Dispatched from St. Louis, MO.

Lead Time: 4-6 business days.

Specifications

Name

Anti-Granzyme B Antibody [CLB-GB11] (FITC)

Description

Mouse monoclonal [CLB-GB11] antibody to Granzyme B (FITC).

Specificity

This antibody recognizes granzyme B, a 31 kDa serine protease expressed intracellularly in activated Tc cells and NK cells.

Applications

Flow Cytometry

Dilutions

Flow Cytometry: The reagent is designed for analysis of human blood cells using 4 µl reagent / 100 µl of whole blood or 10⁶ cells in a suspension. The content of a vial (0.4 ml) is sufficient for 100 tests; Intracellular staining.

Reactivity

Human, Non-Human Primates

Immunogen

Human NK cell line YT-INDY-derived granzyme B.

Host

Mouse

Clonality

Monoclonal

Clone ID

CLB-GB11

Isotype

IgG1

Conjugate

FITC

Excitation: 490nm, Emission: 525nm

Purification

This antibody is conjugated with Fluorescein Isothiocyanate (FITC) under optimum conditions. The conjugate is purified by size-exclusion chromatography; removing unconjugated antibody and free fluorochrome.

Predicted MW

31 kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.4, with 15 mM Sodium Azide.

Storage

Shipped at 4°C. Store in the dark at 2-8°C. Do not freeze!

Synonyms

C11, Cathepsin G-like 1, CGL1, CSPB, CTLA-1, CTLA1, CTSGL1, Cytotoxic T-lymphocyte proteinase 2, Fragmentin-2, GRB, GZMB, Lymphocyte protease

Isotype Controls

Mouse IgG1 [MOPC-21] (FITC) (A86205)

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation DataValidation Data (3)

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Flow Cytometry - Anti-Granzyme B Antibody [CLB-GB11] (FITC) (A254409)

Flow cytometry intracellular staining pattern of human peripheral whole blood stained using Anti-Granzyme B Antibody [CLB-GB11] (FITC) (A254409) (4 µl reagent / 100 µl of peripheral whole blood).

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Flow Cytometry - Anti-Granzyme B Antibody [CLB-GB11] (FITC) (A254409)

Flow cytometry multicolor intracellular staining of human lymphocytes stained using Anti-Granzyme B Antibody [CLB-GB11] (FITC) (A254409) (4 µl reagent / 100 µl of peripheral whole blood) and Anti-CD3 Antibody [UCHT1] (APC) (A86518) (10 µl reagent / 100 µl of peripheral whole blood).

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Flow Cytometry - Anti-Granzyme B Antibody [CLB-GB11] (FITC) (A254409)

Separation of human CD3 negative Granzyme B positive lymphocytes (red-filled) from CD3 negative Granzyme B negative lymphocytes (black-dashed) in flow cytometry analysis (intracellular staining) of human peripheral whole blood stained using Anti-Granzyme B Antibody [CLB-GB11] (FITC) (A254409) (4 µl reagent / 100 µl of peripheral whole blood).

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