Anti-GPCR GPRC5D Antibody [DM89] - BSA and Azide free
Description
Recombinant rabbit monoclonal [DM89] antibody to GPCR GPRC5D.
Applications
ELISA, Flow Cytometry
Dilutions
ELISA: 1:5,000-10,000, Flow Cytometry: 1:100
Reactivity
Human
Host
Rabbit
Clonality
Monoclonal
Clone ID
DM89
Isotype
IgG
Conjugate
Unconjugated
Purification
Affinity chromatography.
Concentration
Reconstitution dependent.
Product Form
Lyophilized
Reconstitution
Reconstitute with distilled sterile water.
Formulation
Lyophilized from sterile Phosphate Buffered Saline, pH 7.4. Normally 5%–8% Trehalose is added as a protectant before lyophilization.
Storage
Shipped at 4°C. Lyophilized: Store at -20°C to -80°C. Reconstituted: Aliquot and store at -80°C. Product is stable for one year. Avoid freeze/thaw cycles.
General Notes
Prior to reconstitution, centrifuge the vial at 5,000g for 3-5 minutes at room temperature. Reconstitute with appropriate volume of distilled sterile water to bring product to 1mg/ml concentration. After addition of distilled sterile water, mix by gentle tapping. Note: It is not recommended to vortex or vigorously pipette the sample.
Synonyms
G-protein coupled receptor family C group 5 member D
Anti-GPCR GPRC5D Antibody [DM89] - BSA and Azide free (A318617)
ELISA plate pre-coated by 2 µg/ml (100 µl/well) Recombinant Human GPCR GPRC5D Protein (Fc Chimera 6xHis Tag) (A318347) can bind Anti-GPCR GPRC5D Antibody [DM89] - Azide free (A318617) in a linear range of 0.256-32 µg/ml.
Expi 293 cell line transfected with irrelevant protein (A) and human GPRC5D (B) were surface stained with Anti-GPCR GPRC5D Antibody [DM89] - Azide free (A318617) at 1 µg/ml followed by Anti-Rabbit IgG Antibody (Alexa 488).
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