Anti-GPCR GPRC5D Antibody [DM62] - BSA and Azide free
Description
Recombinant rabbit monoclonal [DM62] antibody to GPCR GPRC5D.
Applications
ELISA, Flow Cytometry
Dilutions
ELISA: 1:5,000-10,000, Flow Cytometry: 1:100
Reactivity
Human
Host
Rabbit
Clonality
Monoclonal
Clone ID
DM62
Isotype
IgG
Conjugate
Unconjugated
Purification
Affinity chromatography.
Concentration
Reconstitution dependent.
Product Form
Lyophilized
Reconstitution
Reconstitute with distilled sterile water.
Formulation
Lyophilized from sterile Phosphate Buffered Saline, pH 7.4. Normally 5%–8% Trehalose is added as a protectant before lyophilization.
Storage
Shipped at 4°C. Lyophilized: Store at -20°C to -80°C. Reconstituted: Aliquot and store at -80°C. Product is stable for one year. Avoid freeze/thaw cycles.
General Notes
Prior to reconstitution, centrifuge the vial at 5,000g for 3-5 minutes at room temperature. Reconstitute with appropriate volume of distilled sterile water to bring product to 1mg/ml concentration. After addition of distilled sterile water, mix by gentle tapping. Note: It is not recommended to vortex or vigorously pipette the sample.
Synonyms
G-protein coupled receptor family C group 5 member D
Anti-GPCR GPRC5D Antibody [DM62] - BSA and Azide free (A318635)
ELISA plates were pre-coated with Recombinant Human GPCR GPRC5D Protein (Fc Chimera 6xHis Tag) (A318347) at 2 µg/ml (100 µl/well) which can bind Anti-GPCR GPRC5D Antibody [DM62] - BSA and Azide free (A318635) in a linear range of 1-100 ng/ml.
Flow cytometry data of serially titrated Anti-GPCR GPRC5D Antibody [DM62] - BSA and Azide free (A318635) on H929 cells. The Y-axis represents the mean fluorescence intensity (MFI) while the X-axis represents the concentration of IgG used.
Affinity ranking of different Rabbit Anti-GPRC5D Monoclonal Antibody clones by titration of different concentrations onto H929 cells. The Y-axis represents the mean fluorescence intensity (MFI) while the X-axis represents the concentration of IgG used.
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