AF6165 staining NIH-3T3 by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100,then blocked in 10% serum for 45 minutes at 25°C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37°C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) Ab, diluted at 1/600, was used as the secondary antibody.
Western blot analysis of extracts from various samples, using Glycogen Synthase Antibody. Lane 1: HepG2 treated with blocking peptide; Lane 2: HepG2; Lane 3: B16f10; Lane 4: Mouse brain.
