Anti-Glutathione Peroxidase 4 Antibody (A305439)

Western blot analysis of extracts of various cell lines, using Anti-Glutathione Peroxidase 4 Antibody (A305439) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 1s.

Western blot analysis of extracts of Jurkat cells, using Anti-Glutathione Peroxidase 4 Antibody (A305439) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 30s.

Western blot analysis of extracts from wild type (WT) and GPX4 knockdown (KD) U-87MG cells, using Anti-Glutathione Peroxidase 4 Antibody (A305439) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 30s.

Immunofluorescence analysis of mouse testis cells using Anti-Glutathione Peroxidase 4 Antibody (A305439) at a dilution of 1:200 (40x lens). DAPI was used to stain the cell nuclei (blue).