Anti-Gli3 Antibody (A91649)

Name: Anti-Gli3 Antibody
Brand: Antibodies.com
SKU: A91649
Price: 355.0 USD
Availability: InStock

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$355

Rabbit polyclonal antibody to Gli3 for WB and IHC.

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Shipping Information

Freight/Packing Charges: $40

Dispatched from St. Louis, MO.

Lead Time: 7-10 business days.

Specifications

Name

Anti-Gli3 Antibody

Description

Rabbit polyclonal antibody to Gli3.

Applications

WB, IHC

Dilutions

WB: 1:500-1:2,000, IHC: 1:50-1:200

Reactivity

Human, Mouse, Rat

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 800-900 of human GLI3 (NP_000159.3).

Sequence

KAPAVSPLIGNGTQSNNTCSLGGPMTLLPGRSDLSGVDVTMLNMLNRRDSSASTISSAYLSSRRSSGISPCFSSRRSSEASQAEGRPQNVSVADSYDPIST

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Conjugate

Unconjugated

Purification

Affinity purification.

Concentration

Lot Specific

Molecular Weight

250 kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol and 0.01% Thiomersal.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze/thaw cycles.

Synonyms

GLI3 form of 190 kDa, GLI3 full-length protein, GLI3-190, GLI3FL, Transcriptional activator GLI3

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation DataValidation Data (5)

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Western Blot - Anti-Gli3 Antibody (A91649)

Western blot analysis of extracts of various cell lines, using Anti-Gli3 Antibody (A91649) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 3min.

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Immunohistochemistry - Anti-Gli3 Antibody (A91649)

Immunohistochemistry analysis of paraffin-embedded human colon tissue using Anti-Gli3 Antibody (A91649) at a dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

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Immunohistochemistry - Anti-Gli3 Antibody (A91649)

Immunohistochemistry analysis of paraffin-embedded mouse liver using Anti-Gli3 Antibody (A91649) at a dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

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Immunohistochemistry - Anti-Gli3 Antibody (A91649)

Immunohistochemistry analysis of paraffin-embedded rat brain using Anti-Gli3 Antibody (A91649) at a dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.