This antibody recognises green fluorescent protein (GFP) and its variants. GFP is a ~27 kDa protein derived from the jellyfish Aequorea victoria. GFP fluoresces green (509 nm) when excited by blue light (395 nm), and is commonly used as a marker of gene expression. Rabbit anti GFP antibody is known to cross-react with enhanced green fluorescent protein (EGFP), yellow fluorescent protein (YFP) and red fluorescent protein (RFP).
Applications
WB
Dilutions
WB: 0.5 - 4.0 µg/ml
Immunogen
E. coli derived recombinant EGFP.
Host
Rabbit
Clonality
Polyclonal
Isotype
IgG
Conjugate
Unconjugated
Concentration
500 µg/ml
Molecular Weight
Approximately 32-34 kDa on recombinant yellow fluorescent protein (YFP).
Product Form
Liquid
Formulation
Supplied in Phosphate Buffered Saline with 30% Glycerol, 0.5% BSA, and 0.03% ProClin 300.
Storage
Store undiluted at -20°C. Storage in frost free freezers is not recommended. Avoid freeze-thaw cycles. Should this product contain a precipitate we recommend microcentrifugation before use.
General Notes
Rabbit anti GFP antibody recognizes green fluorescent protein (GFP) and its variants. GFP is a ~27 kDa protein derived from the jellyfish Aequorea victoria. GFP fluoresces green (509 nm) when excited by blue light (395 nm), and is commonly used as a marker of gene expression. Rabbit anti GFP antibody is known to cross-react with enhanced green fluorescent protein (EGFP), yellow fluorescent protein (YFP) and red fluorescent protein (RFP).
