Supplied in Phosphate Buffered Saline with 50% Glycerol and 5mM Sodium Azide.
Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
General Notes
This antibody can be used to verify the expression, size, and stability of both AcGFP and eGFP fusion proteins in western blotting experiments, and to amplify GFP signals in tissues of transgenic animals.
Immunofluorescent analysis of transfected HEK293 cells with a GFP-construct in green stained with Anti-GFP Antibody (1:5,000 | red). The blue is Hoechst staining of nuclear DNA. The Anti-GFP Antibody reveals GFP protein expressed only in transfected cells, as a result transfected cells are appeared express both red and green signals and so appear an orange-golden color. Untransfected cells show neither signal.
Western blot analysis of HEK293 cell lysates using Anti-GFP Antibody (1:1,000 | green): [1] protein standard, [2] non-transfected control cells, [3] cells transfected with a GFP construct and [4] cells transfected with an mCherry construct. Strong band at ~27kDa corresponds to GFP protein detected only in cells transfected with GFP construct. This antibody does not recognize the mCherry protein.
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![Western Blot - Anti-GFP Antibody [2D4] (A254386) - Antibodies.com](https://cdn.antibodies.com/image/catalog/254/A254386_1.jpg?profile=product_alternative)