This antibody reacts with GFAP, the principal marker of astroglial cells in the central nervous system, which is specifically expressed in satellite cells in peripheral ganglia and in non myelinating Schwann cells in peripheral nerves. The GFAP protein runs on gels at ~55 kDa protein, usually associated with lower Mw bands which are thought to be proteolytic fragments and alternate transcripts from the single gene.
Applications
IP, WB, IHC-P, ICC
Dilutions
IHC: 1-4 µg/ml, WB: 1-2 µg/ml, IHC-P: 1-2 µg/ml, IP: 1-2 µg / 100-500 µg of protein per 1 ml lysate.
Reactivity
Porcine, Human, Rat
Immunogen
Porcine spinal cord.
Host
Mouse
Clonality
Monoclonal
Clone ID
GA-5
Isotype
IgG1
Conjugate
Unconjugated
Purification
Protein A chromatography.
Concentration
1 mg/ml
Predicted MW
55 kDa
Product Form
Liquid
Formulation
Supplied in Phosphate Buffered Saline, pH 7.4, with 15 mM Sodium Azide.
Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze/thaw cycles.