Anti-GFAP Antibody (A85307)

Immunofluorescent analysis of a section of mouse hippocampus stained with Anti-GFAP Antibody (1:5,000 | green) and co-stained with Anti-FOX3/NeuN Antibody (A85402 | 1:5,000 | red). The blue is DAPI staining of nuclear DNA. Following transcardial perfusion with 4% paraformaldehyde, mouse brain was post fixed for 24 hours, cut to 45 µM, and free-floating sections were stained with the above antibodies. The Anti-GFAP Antibody stains a network of astroglial cells while the Anti-Fox3/NeuN Antibody stains the nuclei and proximal perikarya of neurons.

Mixed cultures of neurons and glia stained with Anti-GFAP Antibody (red) and DNA (blue). Astrocytes stain strongly and specifically in a clearly filamentous fashion with this antibody.

Western blot analysis of whole brain lysates using Anti-GFAP Antibody (1:5,000 | green): [1] protein standard (red), [2] rat brain, [3] mouse brain. The strong band at about 50 kDa corresponds to the GFAP protein. Smaller proteolytic fragments and alternate transcripts of GFAP may also be detected on such blots.