Anti-GFAP Antibody (A270545)

Immunofluorescent analysis of mouse hippocampus section stained with Anti-GFAP Antibody (A270545), at a dilution of 1:5,000, in green. The tissue was co-stained with Anti-Fox3 Antibody (A85404), at a dilution of 1:2,000, in red. Nuclear DNA is visualised in blue using Hoechst staining. Following transcardial perfusion of the mouse with 4% paraformaldehyde, the brain was post-fixed for 24 hours, cut to 45 µm, and free-floating sections were stained with the above antibodies. The Anti-GFAP Antibody (A270545) stains the network of astrocytic glial cells, while the Anti-Fox3 Antibody (A85404) specifically labels nuclei and proximal perikarya of neurons.

Western blot analysis of brain lysates from different species using Anti-GFAP Antibody (A270545), at a dilution of 1:5,000, in green. The lanes contain samples of: [1] Protein standards, in red, [2] rat cortex, [3] rat cerebellum, [4] mouse cortex, [5] mouse cerebellum, [6] cow cortex, and [7] cow cerebellum. The strong band at about 50 kDa corresponds to GFAP protein. Smaller proteolytic fragments of GFAP are also detected on the blot.