Anti-GFAP Antibody [5C10] (A85422)

Immunofluorescent analysis of rat cerebellum section stained with Anti-GFAP Antibody [5C10] (A85422), dilution 1:1,000, in green, co-stained with Anti-NF-L Antibody (A85451), dilution 1:2,000, in red. Following transcardial perfusion with 4% paraformaldehyde, the brain was post-fixed for 24 hours, cut to 45 µm, and free-floating sections were stained with antibodies. Anti-GFAP Antibody [5C10] stains a network of astroglial cells, while the NF-L antibody labels neuronal cells and their processes.

Western blot analysis of whole tissue lysates using Anti-GFAP Antibody [5C10] (A85422), dilution 1:2,000, in green. The lanes contain: [1] protein standard (red), [2] rat brain, [3] rat spinal cord, [4] mouse brain, [5] mouse spinal cord. The strong band at about 50 kDa corresponds to the GFAP protein.