Anti-GFAP Antibody [2A5] (A104314)

Immunofluorescent analysis of an adult rat cerebellum section stained with Anti-GFAP Antibody (1:500 | red) and co-stained with Anti-Parvalbumin Antibody (1:2,000 | green). The blue is DAPI staining of nuclear DNA. Following transcardial perfusion of rat with 4% paraformaldehyde, brain was post fixed for 24 hours, and free-floating 45µM sections were stained with above antibodies. The Anti-GFAP Antibody stains the processes of Bergmann glia and astrocytes. The Anti-Parvalbumin Antibody labels perikarya and dendrites of Purkinje cells and interneurons in the molecular layer of the cerebellum. The staining on rodent tissues is specific but not as robust as on human material.

Western blot analysis of equal amount of total protein from different tissue lysates and recombinant proteins solutions using Anti-GFAP Antibody (1:2,000 | green): [1] protein standard (red), [2] rat brain, [3] rat spinal cord, [4] mouse brain, [5] mouse spinal cord, [6] pig brain, [7] rat recombinant GFAP, [8] human recombinant GFAP. Bands around 50kDa correspond to alternative transcripts and proteolytic products of GFAP. Note that Anti-GFAP Antibody has significantly stronger reactivity with pig and human GFAP as compared to rodent, suggesting that it binds to an epitope which is not totally conserved across mammalian sequences.