Anti-GATA4 Antibody [ARC51718] (A305425)

Name: Anti-GATA4 Antibody [ARC51718]
Brand: Antibodies.com
SKU: A305425
Price: 470.0 USD
Availability: InStock

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$470

Rabbit monoclonal [ARC51718] antibody to GATA4 for WB and IHC.

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Shipping Information

Freight/Packing Charges: $40

Dispatched from St. Louis, MO.

Lead Time: 5-8 business days.

Specifications

Name

Anti-GATA4 Antibody [ARC51718]

Description

Rabbit monoclonal [ARC51718] antibody to GATA4.

Applications

WB, IHC

Dilutions

WB: 1:500-1:1,000, IHC: 1:100-1:500

Reactivity

Human, Mouse, Rat

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 343-442 of human GATA4 (NP_002043.2).

Sequence

ASGASSNSSNATTSSSEEMRPIKTEPGLSSHYGHSSSVSQTFSVSAMSGHGPSIHPVLSALKLSPQGYASPVSQSPQTSSKQDSWNSLVLADSHGDIITA

Host

Rabbit

Clonality

Monoclonal

Clone ID

ARC51718

Isotype

IgG

Conjugate

Unconjugated

Purification

Affinity purification.

Molecular Weight

55 kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol, 0.05% BSA, and 0.05% Proclin 300.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Synonyms

GATA-binding factor 4, Transcription factor GATA-4

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation DataValidation Data (6)

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Western Blot - Anti-GATA4 Antibody [ARC51718] (A305425)

Western blot analysis of extracts of various cell lines, using Anti-GATA4 Antibody [ARC51718] (A305425) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 180s.

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Western Blot - Anti-GATA4 Antibody [ARC51718] (A305425)

Western blot analysis of extracts of Rat liver, using Anti-GATA4 Antibody [ARC51718] (A305425) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Enhanced Kit (RM00021). Exposure time: 90s.

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Immunohistochemistry - Anti-GATA4 Antibody [ARC51718] (A305425)

Immunohistochemistry analysis of paraffin-embedded mouse pancreas using Anti-GATA4 Antibody [ARC51718] (A305425) at a dilution of 1:500(40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

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Immunohistochemistry - Anti-GATA4 Antibody [ARC51718] (A305425)

Immunohistochemistry analysis of paraffin-embedded mouse stomach using Anti-GATA4 Antibody [ARC51718] (A305425) at a dilution of 1:500(40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.