Anti-GATA3 Antibody (A283413)

Anti-GATA3 Antibody

Rabbit polyclonal antibody to GATA3.

This antibody recognises GATA3, also known as Trans-acting T-cell-specific transcription factor GATA-3 or GATA-binding factor 3. The protein is a member of the GATA family of transcription factors, which has received its name from binding DNA sequences containing the GATA consensus motif 5'-[AT]GATA[AG]-3' (uniprot P15976).GATA3 has been shown to play a critical role in T cell development and the differentiation of T helper 2 (Th2) cells. In addition, GATA3 has been implied to have tissue specific functions such as a regulatory role in the development of the mammary gland epithelium and skin tissue. Aberrant GATA3 levels have been identified in a variety of cancers including breast cancer. Indeed, GATA3 has become a marker for certain breast cancer types and GATA3 antibodies are commonly used for the immunohistochemical evaluation of breast cancer samples.

WB, IHC-P, IF, ICC

ICC: 1:50 - 1:500

Human

Purified partial recombinant GATA3 protein expressed in E. coli.

Rabbit

Polyclonal

IgG

Approximately 55 kDa in SH-SY5Y cell lysate and SH-SY5Y nuclear extract

Liquid

Supplied in Phosphate Buffered Saline with 1 mg/ml BSA, 30% Glycerol, and <0.1% Sodium Azide.

Shipped at ambient temperature. Upon delivery aliquot and store at -20°C. When thawed, aliquot the sample as needed. Short term (up to 4 weeks): store at 4°C. Long term: store at -20°C. Avoid freeze / thaw cycles. Storage in frost free freezers is not recommended.

Rabbit anti GATA3 antibody recognizes GATA3, also known as Trans-acting T-cell-specific transcription factor GATA-3 or GATA-binding factor 3. The protein is a member of the GATA family of transcription factors, which has received its name from binding DNA sequences containing the GATA consensus motif 5'-[AT]GATA[AG]-3' (uniprot P15976).GATA3 has been shown to play a critical role in T cell development and the differentiation of T helper 2 (Th2) cells (Ho et al.1991). In addition, GATA3 has been implied to have tissue specific functions such as a regulatory role in the development of the mammary gland epithelium and skin tissue (Chou et al. 2010).Aberrant GATA3 levels have been identified in a variety of cancers including breast cancer. Indeed, GATA3 has become a marker for certain breast cancer types and GATA3 antibodies are commonly used for the immunohistochemical evaluation of breast cancer samples (Krings et al. 2014).

GATA-binding factor 3, Trans-acting T-cell-specific transcription factor GATA-3