Anti-GAPDH Antibody (Biotin) (A83723)

Name: Anti-GAPDH Antibody (Biotin)
Brand: Antibodies.com
SKU: A83723
Price: 480.0 USD
Availability: InStock

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$480

Goat polyclonal antibody to GAPDH (Biotin) for ELISA and WB.

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Alternative Formats and ConjugatesAlternative Formats

Unconjugated

Shipping Information

Freight/Packing Charges: $40

Dispatched from St. Louis, MO.

Lead Time: 3-5 business days.

Tags:

Cytoplasm Marker Cytoskeleton Marker Loading Control Nuclear Marker

Specifications

Name

Anti-GAPDH Antibody (Biotin)

Description

Goat polyclonal antibody to GAPDH (Biotin).

Applications

ELISA, WB

Dilutions

ELISA: 1:64,000, WB: 0.3-1µg/ml

Reactivity

Human, Mouse, Rat

Immunogen

Synthetic peptide corresponding to the C terminal region of human GAPDH.

Sequence

C-HQVVSSDFNSDT.

Host

Goat

Clonality

Polyclonal

Isotype

IgG

Conjugate

Biotin

Purification

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Concentration

500 µg/ml

Molecular Weight

35 kDa

Predicted MW

36.1kDa

Product Form

Liquid

Formulation

Supplied in Tris Buffered Saline, pH 7.3, with 0.5% BSA and 0.02% Sodium Azide.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze/thaw cycles.

Synonyms

GAPD, Glyceraldehyde-3-phosphate dehydrogenase, Peptidyl-cysteine S-nitrosylase GAPDH

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation DataValidation Data (1)

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Western Blot - Anti-GAPDH Antibody (Biotin) (A83723)

GAPDH expression in HEK293 cell lysate analyzed by western blot. Cells were lysed in RIPA buffer and 35µg protein was run per lane. Primary antibody incubation was performed for 1 hour with Anti-GAPDH Antibody (Biotin) (A83723) at 0.5µg/ml and detected by chemiluminescence using horseradish peroxidase conjugated streptavidin and using NAP blocker to substitute for skimmed milk.

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