Western blot analysis of extracts of various cell lines, using Anti-GAPDH Antibody (A92899) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 1s.
Immunohistochemistry analysis of paraffin-embedded rat kidney using Anti-GAPDH Antibody (A92899)Immunohistochemistry analysis of paraffin-embedded rat kidney using GAPDH Rabbit polyclonal antibody (AC027) at a dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
Immunohistochemistry analysis of paraffin-embedded human liver cancer using Anti-GAPDH Antibody (A92899)Immunohistochemistry analysis of paraffin-embedded human liver cancer using GAPDH Rabbit polyclonal antibody (AC027) at a dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
Immunohistochemistry analysis of paraffin-embedded mouse kidney using Anti-GAPDH Antibody (A92899)Immunohistochemistry analysis of paraffin-embedded mouse kidney using GAPDH Rabbit polyclonal antibody (AC027) at a dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
Immunofluorescence analysis of C6 cells using Anti-GAPDH Antibody (A92899) at a dilution of 1:100 (40x lens). DAPI was used to stain the cell nuclei (blue).
Immunofluorescence analysis of NIH-3T3 cells using Anti-GAPDH Antibody (A92899) at a dilution of 1:100 (40x lens). DAPI was used to stain the cell nuclei (blue).
Immunofluorescence analysis of U-2 OS cells using Anti-GAPDH Antibody (A92899) at a dilution of 1:100 (40x lens). DAPI was used to stain the cell nuclei (blue).