Anti-GAPDH Antibody (A85377)

Western blot analysis of HEK293 cell lysates using Anti-mCherry Antibody (A270547), at a dilution of 1:1,000, in green, and Anti-GAPDH Antibody (A85377), at a dilution of 1:5,000, in red. The lanes contain: [Lane 1] protein standard, [Lane 2] untransfected HEK293 control cells, [Lane 3] HEK293 cells transfected with pCI-Neo-mod vector expressing two tdTomato protein domains, [Lane 4] HEK293 cells transfected with pCI-Neo-mod vector expressing one mCherry-HA protein domain, and [Lane 5] HEK293 cells transfected with pCI-Neo-mod vector expressing one GFP domain. Anti-mCherry Antibody (A270547) recognizes tdTomato and mCherry proteins revealing major bands at about 60kDa and 30kDa, but does not recognize GFP. The red band at 37kDa corresponds to GAPDH protein here used as a loading control.

Immunohistochemistry analysis of a NBF fixed paraffin embedded human pancreas section with Anti-GAPDH Antibody (A85377) at a dilution of 1:5,000 detected with DAB (brown) using the Vector Labs ImmPRESS method and reagents with citra buffer retrieval. Counterstained with Hematoxylin (blue). The GAPDH antibody labels pancreatic exocrine glandular cells as well as a sub-population of endocrine cells. Note: this antibody performs well in testing with both 4% PFA and standard NBF fixed human, mouse, and rat tissues.