Anti-GAPDH Antibody (A85377)

HeLa cells stained with Anti-GAPDH Antibody (red), Anti-TAF15 Antibody (A85450 | green) and DNA (blue). The Anti-GAPDH Antibody reveals strong cytoplasmic staining, while the Anti-TAF15 Antibody reveals a granular nuclear localization.

Immunofluorescent analysis of HeLa cells stained with Anti-GAPDH Antibody (A85377), at a dilution of 1:2,000 in red. Blue is Hoechst staining of nuclear DNA. The Anti-GAPDH Antibody (A85377) produces diffuse cytoplasmic staining of cells.

Western blot analysis of different cell cytosolic or nuclear enriched fractions, using Anti-GAPDH Antibody (A85377), at a dilution of 1:20,000, in red. The lanes contain samples of: [1] Protein standards, in red, [2] NIH-3T3 cytosolic, [3] NIH-3T3 nuclear, [4] HeLa cytosolic, and [5] HeLa nuclear fractions. Strong band at 37kDa corresponds to GAPDH protein, mainly detected in the cytosolic fractions. The same blot was simultaneously probed with Anti-SF3B4 Antibody (A85417), at a dilution of 1:1,000, in green. In contrast to GAPDH, SF3B4 is exclusively expressed in the nuclear fraction.

Blot of HeLa cells probed with Anti-GAPDH Antibody. Note the single clean band at 36 kDa corresponding to GAPDH.