Synthetic peptide within amino acids 300 and the C-terminus of human GAPDH (NP_002037. 2).
Host
Rabbit
Clonality
Polyclonal
Isotype
IgG
Conjugate
Unconjugated
Purification
Antigen affinity purification.
Concentration
200 µg/ml
Product Form
Liquid
Formulation
Supplied in Tris-Buffered Saline with 0.1% BSA and 0.09% Sodium Azide.
Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze/thaw cycles.
General Notes
This antibody was affinity purified using the immunising peptide immobilized on solid support. Immunoglobulin concentration was determined by extinction coefficient: absorbance at 280 nm of 1. 4 equals 1.0 mg of IgG.
Samples: Whole cell lysate (50 µg) from NIH 3T3, CT26, CH27, TCMK-1, and BW5147.3 cells prepared using NETN lysis buffer. Antibody: Anti-GAPDH Antibody (A295702) was used for WB at 0.04 µg/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.
Samples: Whole cell lysate (10 µg) from HeLa, Jurkat, RKO, U2OS, and A-549 cells prepared using NETN lysis buffer. Antibody: Anti-GAPDH Antibody (A295702) was used for WB at 0.04 µg/ml. Detection: Chemiluminescence with an exposure time of 3 seconds.
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