Anti-GAPDH Antibody [1D4] (A85382)

Western blot analysis of different cell line lysates using Anti-MARCKS Antibody (A104335), at a dilution of 1:1,000, in green. The lanes contain: [Lane 1] protein standard (red), [Lane 2] HEK293 cells, [Lane 3] HeLa cells, [Lane 4] SH-SY5Y cells, and [Lane 5] COS1 cells. Strong bands at 80kDa corresponds to MARCKS protein. The same blot was simultaneously probed with Anti-GAPDH Antibody [1D4] (A85382), at a dilution of 1:5,000, in red, which reveals a single band at 37kDa in all preparations.

Western blot analysis of different tissue and cell lysates using Anti-SERT Antibody (A104336), at a dilution of 1:1,000, in green. Left: [Lane 1] protein standard, [Lane 2] rat brain caudate/putmen region, [Lane 3] rat brain striatum region, and [Lane 4] mouse brain. The prominent band at ~68kDa corresponds to SERT protein, and lower bands are likely proteolytic products of SERT. Right: [Lane 5] protein standard, [Lane 6] non-transfected HEK293 cells, and [Lane 7] HEK293 cells transfected with an expression construct containing a Myc-DDK tagged full-length human SERT cDNA. The bands between 50-75kDa in the transfected cells demonstrate overexpression of SERT, and the band at ~150kDa presumably corresponds to an aggregated form of SERT. The blot was simultaneously probed with Anti-GAPDH Antibody [1D4] (A85382), at a dilution of 1:5,000, in red, which reveals the single band at ~37kDa in both transfected and non-transfected cells. Note that HEK293 cells express SERT endogenously.

Immunohistochemistry analysis of a NBF fixed paraffin embedded human pancreas section with Anti-GAPDH Antibody [1D4] (A85382) at a dilution of 1:1,000 detected with DAB (brown) using the Vector labs Elite ABC-HRP detection and goat anti-mouse IgM secondary with citra buffer retrieval. Counterstained with Hematoxylin (blue). Anti-GAPDH Antibody [1D4] (A85382) strongly labels pancreatic exocrine glandular cells. Note: this antibody performs well in testing with both 4% PFA and standard NBF fixed tissues.