The epitope for the antibody is located within the peptide KYDDIKKVVKQASEGPLKGI, amino acids 254-273 of human GAPDH. This peptide is highly conserved across mammalian species.
Applications
WB, ICC/IF, IHC
Dilutions
WB: 1:1,000, ICC/IF: 1:100, IHC: 1:100
Reactivity
Human, Rat, Mouse, Bovine, Porcine, Horse, Monkey, Canine, Chicken
Immunogen
Native full-length GAPDH protein, purified from pig blood cells.
Host
Mouse
Clonality
Monoclonal
Clone ID
1D4
Isotype
IgM
Conjugate
Unconjugated
Purification
Immunogen affinity purification.
Concentration
1 mg/ml
Molecular Weight
36 kDa
Product Form
Liquid
Formulation
Supplied in Phosphate Buffered Saline with 50% Glycerol and 5mM Sodium Azide.
Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Immunofluorescent analysis of HeLa cells stained with Anti-GAPDH Antibody [1D4] (A85382), dilution 1:100, in green. Blue is Hoechst staining of nuclear DNA. The anti-GAPDH antibody produces strong cytoplasmic staining of healthy cells.
Western blot analysis of cell line lysates probed with Anti-GAPDH Antibody [1D4] (A85382), dilution 1:2,000. The lanes contain: [1] protein standard, [2] HEK293, [3] HeLa, [4] SH-SY5Y, [5] COS1, [6] NIH-3T3, and [7] C6 cell lysates. The GAPDH antibody reveals a single band at ~37 kDa in all cell lines. GAPDH is a 'house keeping' protein, the level of which is relatively unaffected by most experimental manipulations, and, as a result, this antibody has been widely used as a western blot loading control.
Publishing research using Anti-GAPDH Antibody [1D4] (A85382)? Please let us know so that we can list the citation on this page.
![Immunofluorescence - Anti-GAPDH Antibody [1D4] (A85382) - Antibodies.com](https://cdn.antibodies.com/image/catalog/85/A85382_1.jpg?profile=product_top)
![Western Blot - Anti-GAPDH Antibody [1D4] (A85382) - Antibodies.com](https://cdn.antibodies.com/image/catalog/85/A85382_2.jpg?profile=product_top)
![Immunofluorescence - Anti-GAPDH Antibody [1D4] (A85382) - Antibodies.com](https://cdn.antibodies.com/image/catalog/85/A85382_1.jpg?profile=product_top_thumb)
![Western Blot - Anti-GAPDH Antibody [1D4] (A85382) - Antibodies.com](https://cdn.antibodies.com/image/catalog/85/A85382_2.jpg?profile=product_top_thumb)
![Immunofluorescence - Anti-GAPDH Antibody [1D4] (A85382) - Antibodies.com](https://cdn.antibodies.com/image/catalog/85/A85382_1.jpg?profile=product_image)
![Western Blot - Anti-GAPDH Antibody [1D4] (A85382) - Antibodies.com](https://cdn.antibodies.com/image/catalog/85/A85382_2.jpg?profile=product_image)
![Western Blot - Anti-GAPDH Antibody [ARC50888] (A309068) - Antibodies.com](https://cdn.antibodies.com/image/catalog/309/A309068_1.jpg?profile=product_alternative)
![Immunocytochemistry - Anti-GAPDH Antibody [RM114] (A121303) - Antibodies.com](https://cdn.antibodies.com/image/catalog/121/A121396_1.png?profile=product_alternative)
