Anti-GAP43 Antibody (A85394)

Immunofluorescent analysis of cortical neuron-glial cell culture from E20 rat stained with Anti-GAP43 Antibody (1:2,000 | green) and Anti-Vimentin Antibody (A85423 | 1:2,000 | red). The blue is DAPI staining of nuclear DNA. Anti-GAP43 Antibody labels protein expressed in the axonal membrane of neuronal cells, while Anti-Vimentin Antibody stains intermediate filaments in fibroblasts and other non-neuronal cells.

Mixed neuron-glial cultures stained with Anti-GAP43 Antibody (red). Blue is DNA staining.

Western blot analysis of different tissue and cell lysates using Anti-GAP43 Antibody (1:20,000 | green): [1] protein standard (red), [2] rat brain, [3] rat spinal cord, [4] mouse brain, [5] mouse spinal cord, [6] SH-SY5Y cells, [7] C6 cells. Single band at 43 kDa mark corresponds to GAP43 protein. The GAP43 protein is detected only in the lysates of neuronal origin. C6 cells are a rat glioma cell line and do not express GAP43 protein.

Western blot of homogenate of cow cerebellum stained with Anti-GAP43 Antibody. A prominent band running at ~43 kDa represents the full length GAP43.