Anti-GAP43 Antibody (A85292)

Mixed neuron-glial cultures stained with Anti-GAP43 Antibody (green) and Anti- rabbit antibody to alpha-II spectrin (A85352 | red), and DNA (blue). The Anti-GAP43 Antibody stains numerous axonal and dendritic profiles in these cultures, clearly revealing the submembraneous cytoskeleton and vesicles.

Western blot analysis of different tissue and cell lysates using Anti-GAP43 Antibody (1:5,000 | green): [1] protein standard (red), [2] rat brain, [3] rat spinal cord, [4] mouse brain, [5] mouse spinal cord, [6] C6 cells, [7] SH-SY5Y cells. Single band at the 43kDa mark corresponds to GAP43. The GAP43 protein only is detected in the lysates of neuronal origin. C6 cells are a rat glioma cell line and do not express GAP43.

Western blots of extract of cow hippocampus stained with Anti-GAP43 Antibody. A prominent band running at ~43 kDa represents GAP43.