Anti-GAP43 Antibody [5E8] (A85392)

Immunofluorescent analysis of cortical neuron-glial cell culture from E20 rat stained with Anti-GAP43 Antibody, at a dilution of 1:1,000, in red, and Anti-GFAP Antibody (A85307 | 1:2,000, in green. The blue is DAPI staining of nuclear DNA. Anti-GAP43 Antibody labels protein expressed in the axonal membrane of the neuronal cells and Anti-GFAP Antibody stains astroglia.

Mixed rat neuronal cultures stained with Anti-GAP43 Antibody (green). The Anti-GAP43 Antibody stains the plasma membrane of neurons and is particularly concentrated in dendrites.

Western blot analysis of different tissue and cell lysates using Anti-GAP43 Antibody, at a dilution of 1:2,000, in green,: [Lane 1] protein standard (red), [Lane 2] rat brain, [Lane 3] rat spinal cord, [Lane 4] mouse brain, [Lane 5] mouse spinal cord, [Lane 6] C6 cells, [Lane 7] SH-SY5Y cells. Single band at 43 kDa mark corresponds to GAP43 protein. The protein is expressed in rodent and human neurons and neuronal derived cells but not in C6 cells which are of glial origin.

Immunohistochemistry analysis of a formalin fixed paraffin embedded human brain cortex section with Anti-GAP43 Antibody [5E8] (A85392) at a dilution of 1:1,000 detected with DAB (brown) using the Vector Labs ImmPRESS method and reagents with citra buffer retrieval. Counterstained with Hematoxylin (blue). Anti-GAP43 Antibody [5E8] (A85392) labels neurons (and to a lesser degree reactive glial cells) and is concentrated in growth cones and axon terminals. Note: this antibody performs well in testing with 4% PFA or standard NBF fixed human and rat tissue. For better performance in staining long term fixed material, Anti-GAP43 Antibody [1E3] (A85391) is the recommended and preferred clone for GAP43 paraffin staining applications.