Anti-GAP43 Antibody [1E3] (A85391)

Name: Anti-GAP43 Antibody [1E3]
Brand: Antibodies.com
SKU: A85391
Price: 190.0 USD
Availability: InStock

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$190

Mouse monoclonal [1E3] antibody to GAP43 for WB, ICC/IF and IHC.

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Shipping Information

Freight/Packing Charges: $40

Dispatched from St. Louis, MO.

Lead Time: 5-8 business days.

Tags:

Neuronal Marker Schwann Cell Marker

Specifications

Name

Anti-GAP43 Antibody [1E3]

Description

Mouse monoclonal [1E3] antibody to GAP43.

Applications

WB, ICC/IF, IHC

Dilutions

WB: 1:10,000, ICC/IF: 1:1,000, IHC: 1:1,000

Reactivity

Human, Rat, Mouse

Immunogen

C-terminal peptide of rat GAP43, with an N-terminal Cys added to allow chemical coupling to the KLH carrier protein.

Sequence

KEDPEADQEHA

Host

Mouse

Clonality

Monoclonal

Clone ID

1E3

Isotype

IgG1

Conjugate

Unconjugated

Purification

Immunogen affinity purification.

Concentration

1 mg/ml

Molecular Weight

38 kDa, 43 kDa (by SDS-PAGE)

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline with 50% Glycerol and 5mM Sodium Azide.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze/thaw cycles.

General Notes

This antibody binds strongly to growth cones and axonal processes of neurons in cell culture and to synaptic regions in sectioned material.

Synonyms

Axonal membrane protein GAP-43, Growth-associated protein 43, Neural phosphoprotein B-50, Neuromodulin, pp46

Isotype Controls

Suitable Secondaries

See all Anti-Mouse IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation DataValidation Data (5)

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Immunofluorescence - Anti-GAP43 Antibody [1E3] (A85391)

Immunofluorescent analysis of mixed neuronal cultures, co-stained with Anti-GAP43 Antibody [1E3] (A85391) (green), a rabbit antibody to microtubule associated protein 2 (MAP2) (red), and a stain for DNA (blue). The GAP43 antibody stains the plasma membrane of neurons and is particularly concentrated in dendrites.

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Western Blot - Anti-GAP43 Antibody [1E3] (A85391)

Western blot of whole rat spinal cord lysates probed with Anti-GAP43 Antibody [1E3] (A85391) to GAP43 in lane 16 and another similar antibody in lane 17. Dots in middle of strips indicate position of 50kDa and 37kDa protein bands. The strong single band running at about 43kDa corresponds to GAP43.

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Immunofluorescence - Anti-GAP43 Antibody [1E3] (A85391)

Mixed neuronal cultures stained with Anti-GAP43 Antibody (green), Anti-MAP2 Antibody (A85363 | red) and DNA (blue). The Anti-GAP43 Antibody stains the plasma membrane of neurons and is particularly concentrated in dendrites.

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Western Blot - Anti-GAP43 Antibody [1E3] (A85391)

Western blot of whole rat spinal cord lysates probed with Anti-GAP43 Antibody in Lane 16 and another similar antibody in Lane 17. Dots in middle of strips indicate position of 50kDa and 37kDa protein bands. Note that the strong single band running at about 43kDa corresponds to GAP43.