Western Blot - Anti-gamma Tubulin Antibody [ARC1684] (A306765)
Western blot analysis of extracts of various cell lines, using Anti-gamma Tubulin Antibody [ARC1684] (A306765) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 3s.
Immunohistochemistry analysis of paraffin-embedded rat testis using Anti-gamma Tubulin Antibody [ARC1684] (A306765) at a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Immunohistochemistry analysis of paraffin-embedded human thyroid cancer using Anti-gamma Tubulin Antibody [ARC1684] (A306765) at a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Immunohistochemistry analysis of paraffin-embedded mouse testis using Anti-gamma Tubulin Antibody [ARC1684] (A306765) at a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Confocal imaging of NIH-3T3 cells using Anti-gamma Tubulin Antibody [ARC1684] (A306765), at a dilution of 1:100, (red). The cells were counterstained with Alpha-tubulin (ubiquitous) chain Rabbit mAb, at a dilution of 1:100, (green). DAPI was used for nuclear staining (Blue). Objective: 60x.
Confocal imaging of U-2OS cells using Anti-gamma Tubulin Antibody [ARC1684] (A306765), at a dilution of 1:100, (green). The cells were counterstained with Anti-alpha Tubulin Antibody, at a dilution of 1:100, (red). DAPI was used for nuclear staining (Blue). Objective: 60x.