Immunofluorescent analysis of cortical neuron-glial cell culture from E20 rat stained with Anti-Galectin 3 Antibody (1:2,000 | green), and co-stained with Anti-GFAP Antibody (1:2,000 | red). The blue is Hoechst staining of nuclear DNA. Certain glial cells express galectin-3 protein and so appear green, while the majority of glial cells and astrocytes produce GFAP protein and so appear red, and a few cells that express both proteins appear orange-yellow.
Immunofluorescent analysis of cortical neuron-glial cell culture from E20 rat stained with Anti-Galectin 3 Antibody (1:2,000 | green), and co-stained with Anti-MAP2 Antibody [4H5] (A85297) (1:2,000 | red). The blue is Hoechst staining of nuclear DNA. Anti-Galectin 3 Antibody produces strong staining of certain glial cells, while Anti-MAP2 Antibody [4H5] labels dendrites and perikarya of mature neurons.
Western blot analysis of different tissue and cell lysates using Anti-Galectin 3 Antibody (1:5,000 | green): [1] protein standard and mouse tissue lysates: [2] heart, [3] liver, [4] kidney, [5] lung, [6] rat cortical neuron-glial primary cell culture lysate and [7] pig spinal cord lysate. The band at about 30kDa corresponds to the galectin 3 protein
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