Anti-GAL4 Antibody [ARC2073] (A307096)

Name: Anti-GAL4 Antibody [ARC2073]
Brand: Antibodies.com
SKU: A307096
Price: 485.0 USD
Availability: InStock

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$485

Rabbit monoclonal [ARC2073] antibody to GAL4 for WB, IHC, ICC/IF and IP.

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Shipping Information

Freight/Packing Charges: $40

Dispatched from St. Louis, MO.

Lead Time: 7-10 business days.

Specifications

Name

Anti-GAL4 Antibody [ARC2073]

Description

Rabbit monoclonal [ARC2073] antibody to GAL4.

Applications

WB, IHC, ICC/IF, IP

Dilutions

WB: 1:500-1:2,000, IHC: 1:50-1:200, ICC/IF: 1:50-1:200, IP: 1:500-1:1,000

Reactivity

Human, Mouse, Rat

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 1-100 of human Galectin 4/LGALS4 (P56470).

Sequence

MAYVPAPGYQPTYNPTLPYYQPIPGGLNVGMSVYIQGVASEHMKRFFVNFVVGQDPGSDVAFHFNPRFDGWDKVVFNTLQGGKWGSEERKRSMPFKKGAA

Host

Rabbit

Clonality

Monoclonal

Clone ID

ARC2073

Isotype

IgG

Conjugate

Unconjugated

Purification

Affinity purification.

Concentration

Lot Specific

Molecular Weight

35 kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol, 0.05% BSA, and 0.02% Sodium Azide.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze/thaw cycles.

Synonyms

Antigen NY-CO-27, Gal-4, Galectin-4, L-36 lactose-binding protein, L36LBP, Lactose-binding lectin 4, LGALS4

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation DataValidation Data (7)

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Western Blot - Anti-GAL4 Antibody [ARC2073] (A307096)

Western blot analysis of extracts of various cell lines, using Anti-GAL4 Antibody [ARC2073] (A307096) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 30s.

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Immunohistochemistry - Anti-GAL4 Antibody [ARC2073] (A307096)

Immunohistochemistry analysis of paraffin-embedded human appendix tissue using Anti-GAL4 Antibody [ARC2073] (A307096) at a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

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Immunohistochemistry - Anti-GAL4 Antibody [ARC2073] (A307096)

Immunohistochemistry analysis of paraffin-embedded mouse colon using Anti-GAL4 Antibody [ARC2073] (A307096) at a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

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Immunofluorescence - Anti-GAL4 Antibody [ARC2073] (A307096)

Immunofluorescence analysis of rat rectum using Anti-GAL4 Antibody [ARC2073] (A307096) at a dilution of 1:100 (40x lens). DAPI was used to stain the cell nuclei (blue).