This antibody recognises both molecular forms of glutamate decarboxylase, GAD1 (GAD67) and GAD2 (GAD65), the enzyme which converts glutamic acid to gamma -aminobutyric acid (GABA), the major inhibitory transmitter in the higher brain region.
Applications
WB, IHC-P, IHC-Fr, ELISA, IF
Dilutions
IHC-Fr: 1:200 - 1:5,000, WB: 1:1,000 - 1:10,000
Reactivity
Rat, Cat, Human, Mouse
Immunogen
Synthetic peptide sequence DFLIEEIERLGQDL from the C terminus of rat GAD2.
Sequence
DFLIEEIERLGQDL
Host
Rabbit
Clonality
Polyclonal
Isotype
IgG
Conjugate
Unconjugated
Concentration
Lot Specific
Molecular Weight
Detects a double band at approximately 65/67 kDa can be expected
Product Form
Liquid
Formulation
Supplied in Phosphate Buffered Saline with 0.09% Sodium Azide.
Storage
Shipped at ambient temperature. Upon delivery aliquot and store at -20°C. When thawed, aliquot the sample as needed. Short term (up to 4 weeks): store at 4°C. Long term: store at -20°C. Avoid freeze / thaw cycles. Storage in frost free freezers is not recommended.
General Notes
Rabbit anti Glutamate Decarboxylase 1/2 antibody recognizes both molecular forms of glutamate decarboxylase, GAD1 (GAD67) and GAD2 (GAD65), the enzyme which converts glutamic acid to gamma -aminobutyric acid (GABA), the major inhibitory transmitter in the higher brain region.