GAD65 expression in Human Brain (Hippocampus) lysate analyzed by western blot. Cells were lysed in RIPA buffer and 35µg protein was run per lane. Primary antibody incubation was performed with Anti-GAD65 Antibody (A83263) at 0.5µg/ml and detected by chemiluminescence.
GAD65 expression in Human Cortex analyzed by immunohistochemistry. Tissue was paraffin-embedded, and antigen retrieval was achieved by heating in citrate buffer, pH 6. Staining was performed with Anti-GAD65 Antibody (A83263) at 6µg/ml and revealed with horseradish peroxidase (HRP).
Negative control for GAD65 expression in Human Cortex analyzed by immunohistochemistry. Tissue was paraffin-embedded, and staining procedure was performed in the absence of primary antibody.
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