Anti-GAD65 Antibody (A12729)

Name: Anti-GAD65 Antibody
Brand: Antibodies.com
SKU: A12729
Price: 355.0 USD
Availability: InStock

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$355

Rabbit polyclonal antibody to GAD65 for WB and ICC/IF.

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Shipping Information

Freight/Packing Charges: $40

Dispatched from St. Louis, MO.

Lead Time: 7-10 business days.

Tags:

Neuronal Marker

Specifications

Name

Anti-GAD65 Antibody

Description

Rabbit polyclonal antibody to GAD65.

Applications

WB, ICC/IF

Dilutions

WB: 1:500-1:1,000, ICC/IF: 1:50-1:200

Reactivity

Human, Mouse, Rat

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 401-585 of human GAD65/GAD2 (NP_001127838.1).

Sequence

PLQCSALLVREEGLMQNCNQMHASYLFQQDKHYDLSYDTGDKALQCGRHVDVFKLWLMWRAKGTTGFEAHVDKCLELAEYLYNIIKNREGYEMVFDGKPQHTNVCFWYIPPSLRTLEDNEERMSRLSKVAPVIKARMMEYGTTMVSYQPLGDKVNFFRMVISNPAATHQDIDFLIEEIERLGQDL

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Conjugate

Unconjugated

Purification

Affinity purification.

Concentration

Lot Specific

Molecular Weight

60 kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol and 0.01% Thiomersal.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze/thaw cycles.

Synonyms

65 kDa glutamic acid decarboxylase, GAD-65, GAD2, Glutamate decarboxylase 2, Glutamate decarboxylase 65 kDa isoform

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation DataValidation Data (2)

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Western Blot - Anti-GAD65 Antibody (A12729)

Western blot analysis of extracts of various cell lines, using Anti-GAD65 Antibody (A12729) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 5s.

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Immunofluorescence - Anti-GAD65 Antibody (A12729)

Immunofluorescence analysis of rat brain cells using Anti-GAD65 Antibody (A12729) at a dilution of 1:100 (40x lens). DAPI was used to stain the cell nuclei (blue).

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