Anti-Fyn Antibody (A309065)

Name: Anti-Fyn Antibody
Brand: Antibodies.com
SKU: A309065
Price: 355.0 USD
Availability: InStock

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$355

Rabbit polyclonal antibody to Fyn for WB, IHC and ICC/IF.

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Shipping Information

Freight/Packing Charges: $40

Dispatched from St. Louis, MO.

Lead Time: 7-10 business days.

Specifications

Name

Anti-Fyn Antibody

Description

Rabbit polyclonal antibody to Fyn.

Applications

WB, IHC, ICC/IF

Dilutions

WB: 1:100-1:500, IHC: 1:50-1:200, ICC/IF: 1:50-1:200

Reactivity

Human, Mouse, Rat

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 200-300 of human Fyn (NP_002028.1).

Sequence

VKHYKIRKLDNGGYYITTRAQFETLQQLVQHYSERAAGLCCRLVVPCHKGMPRLTDLSVKTKDVWEIPRESLQLIKRLGNGQFGEVWMGTWNGNTKVAIKT

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Conjugate

Unconjugated

Purification

Affinity purification.

Concentration

Lot Specific

Molecular Weight

61 kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol and 0.01% Thiomersal.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze/thaw cycles.

Synonyms

p59-Fyn, Proto-oncogene c-Fyn, Proto-oncogene Syn, SLK, Src-like kinase, Tyrosine-protein kinase Fyn

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation DataValidation Data (5)

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Western Blot - Anti-Fyn Antibody (A309065)

Western blot analysis of extracts of various cell lines, using Anti-Fyn Antibody (A309065) at 1:500 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 180s.

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Western Blot - Anti-Fyn Antibody (A309065)

Western blot analysis of extracts of Rat brain, using Anti-Fyn Antibody (A309065) at 1:500 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Enhanced Kit (RM00021). Exposure time: 180s.

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Immunohistochemistry - Anti-Fyn Antibody (A309065)

Immunohistochemistry analysis of paraffin-embedded human placenta using Anti-Fyn Antibody (A309065) at a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

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Immunofluorescence - Anti-Fyn Antibody (A309065)

Immunofluorescence analysis of Jurkat cells using Anti-Fyn Antibody (A309065) at a dilution of 1:100 (40x lens). DAPI was used to stain the cell nuclei (blue).