Rabbit polyclonal antibody to FRS2 (Phospho-Tyr436)
Specificity
The antibody detects endogenous levels of FRS2 only when phosphorylated at tyrosine 436.
Applications
WB, IHC
Dilutions
Western blotting: 1:500~1:1000Immunohistochemistry: 1:50~1:100
Reactivity
Human
Immunogen
Peptide sequence around phosphorylation site of tyrosine 436(L-N-Y(p)-I-Q) derived from Human FRS2 .
Host
Rabbit
Clonality
Polyclonal
Conjugate
Unconjugated
Purification
Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
Concentration
1 mg/ml
Formulation
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Western blot analysis of extracts from HuvEc cells (Lane 2) and JK cells (Lane 3), using FRS2 (Phospho-Tyr436) Antibody #11769. The lane on the left is treated with antigen-specific peptide.
Immunohistochemical analysis of paraffin-embedded human brain tissue using FRS2 (Phospho-Tyr436) antibody #11769 (left)or the same antibody preincubated with blocking peptide (right).
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