Anti-Frizzled 4 Antibody [10E10] - BSA and Azide free
Description
Recombinant chimeric monoclonal [10E10] antibody to Frizzled 4.
Applications
Flow Cytometry
Dilutions
Flow Cytometry: 1:100
Reactivity
Human
Host
Rabbit / Human
Clonality
Monoclonal
Clone ID
10E10
Isotype
IgG1
Conjugate
Unconjugated
Purification
Affinity chromatography.
Concentration
Reconstitution dependent.
Product Form
Lyophilized
Reconstitution
Prior to reconstitution, centrifuge the vial at 5,000g for 3-5 minutes at room temperature. Reconstitute with appropriate volume of distilled sterile water to bring product to 1mg/ml concentration. After addition of distilled sterile water, mix by gentle tapping. Note: It is not recommended to vortex or vigorously pipette the sample.
Formulation
Lyophilized from sterile Phosphate Buffered Saline, pH 7.4. Normally 5 % - 8% trehalose is added as protectants before lyophilization.
Storage
Shipped at 4°C. Lyophilized: Store at -20°C to -80°C. Reconstituted: Aliquot and store at -80°C. Product is stable for one year. Avoid freeze/thaw cycles.
General Notes
This antibody is a recombinant rabbit chimeric monoclonal antibody containing human IgG1 Fc.
Flow cytometry analysis of 1µg/ml of Anti-Frizzled 4 Antibody [10E10] - BSA and Azide free (A324646) on Expi293 cells transfected with human FZD4 (Blue histogram) or Expi293 transfected with irrelevant protein (Red histogram).
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