FOXO3A expression in Human Heart lysate with (A) and without (B) immunising peptide blockage analyzed by western blot. Cells were lysed in RIPA buffer and 35µg protein was run per lane. Primary antibody incubation was performed with Anti-FOXO3A Antibody (A83729) at 0.3µg/ml and detected by chemiluminescence.
FOXO3A expression in Human Kidney analyzed by immunohistochemistry. Tissue was paraffin-embedded, and antigen retrieval was achieved by heating in citrate buffer, pH 6. Staining was performed with Anti-FOXO3A Antibody (A83729) at 5µg/ml and revealed with horseradish peroxidase (HRP).
Negative control for FOXO3A expression in Human Kidney analyzed by immunohistochemistry. Tissue was paraffin-embedded, and staining procedure was performed in the absence of primary antibody.
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