The antibody detects endogenous levels of FOXO1 / 3 / 4-pan only when phosphorylated at threonine 24 / 32.
Applications
WB
Dilutions
Western blotting: 1:500~1:1000
Reactivity
Human, Mouse
Immunogen
Peptide sequence around phosphorylation site of threonine 24 / 32(S-C-T(p)-W-P) derived from Human FOXO1 / 3 / 4-pan .
Host
Rabbit
Clonality
Polyclonal
Conjugate
Unconjugated
Purification
Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
Concentration
1 mg/ml
Formulation
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage
Store at -20˚C / 1 year
Synonyms
FKHR, Forkhead box protein O1, Forkhead box protein O1A, Forkhead in rhabdomyosarcoma, FOXO1A
Western blot analysis of extracts from 293 cells treated with Serum using FOXO1/3/4-pan (Phospho-Thr24/32) Antibody #11660.The lane on the right is treated with the antigen-specific peptide.
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