Anti-FOXA1 Antibody (A83938)

FOXA1 expression in HepG2 (A) and MCF7 (B) nuclear cell lysate analyzed by western blot. Cells were lysed in RIPA buffer and 35µg protein was run per lane. Primary incubation was performed with Anti-FOXA1 Antibody (A83938) at 0.1µg/ml (A) or 0.3µg/ml (B) and detected by chemiluminescence.

FOXA1 expression in Mouse Liver lysate analyzed by western blot. Cells were lysed in RIPA buffer and 35µg protein was run per lane. Primary antibody incubation was performed with Anti-FOXA1 Antibody (A83938) at 0.1µg/ml and detected by chemiluminescence.

FOXA1 expression in MCF7 cells analyzed by immunofluorescence. Cells were permeabilized with 0.15% Triton. Staining was performed with Anti-FOXA1 Antibody (A83938) at 10µg/ml for 1 hour and Alexa Fluor 488 secondary antibody at 2µg/ml. Strong nuclear staining shown and nuclei were stained with DAPI (blue) while actin filaments were stained with phalloidin (red). Negative control: Goat IgG Isotype Control at 10µg/ml followed by Alexa Fluor 488 secondary antibody at 2µg/ml.

FOXA1 expression in U2OS cells analyzed by immunofluorescence. Cells were permeabilized with 0.15% Triton. Staining was performed with Anti-FOXA1 Antibody (A83938) at 10µg/ml for 1 hour and Alexa Fluor 488 secondary antibody at 2µg/ml. Strong nuclear staining shown and nuclei were stained with DAPI (blue) while actin filaments were stained with phalloidin (red). Negative control: Goat IgG Isotype Control at 10µg/ml followed by Alexa Fluor 488 secondary antibody at 2µg/ml.