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Anti-Flt3/CD135 Antibody (A88079)

Western Blot - Anti-Flt3/CD135 Antibody (A88079) - Antibodies.com Immunohistochemistry - Anti-Flt3/CD135 Antibody (A88079) - Antibodies.com Immunohistochemistry - Anti-Flt3/CD135 Antibody (A88079) - Antibodies.com
$355
Product Datasheet

Rabbit polyclonal antibody to Flt3/CD135 for WB and IHC.

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Shipping Information

$40
Dispatched from St. Louis, MO.
Lead Time: 7-10 business days.

  • Specifications

Name
Anti-Flt3/CD135 Antibody
Description
Rabbit polyclonal antibody to Flt3/CD135.
Applications
WB, IHC
Dilutions
WB: 1:100-1:500, IHC: 1:50-1:200
Reactivity
Human, Mouse, Rat
Immunogen
A synthetic peptide corresponding to a sequence within amino acids 650-750 of human FLT3 (NP_004110.2).
Sequence
ADSSEREALMSELKMMTQLGSHENIVNLLGACTLSGPIYLIFEYCCYGDLLNYLRSKREKFHRTWTEIFKEHNFSFYPTFQSHPNSSMPGSREVQIHPDSD
Host
Rabbit
Clonality
Polyclonal
Isotype
IgG
Conjugate

Unconjugated

Purification
Affinity purification.
Concentration
Lot Specific
Molecular Weight
130 kDa / 160 kDa
Product Form
Liquid
Formulation
Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol and 0.02% Sodium Azide.
Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze/thaw cycles.
Isotype Controls
Suitable Secondaries
See all Anti-Rabbit IgG Secondaries →
Disclaimer
This product is for research use only. It is not intended for diagnostic or therapeutic use.

  • Scientific Validation DataValidation Data (3)

Western Blot - Anti-Flt3/CD135 Antibody (A88079) - Antibodies.comEnlarge Image

Western Blot - Anti-Flt3/CD135 Antibody (A88079)

Western blot analysis of various lysates, using Anti-Flt3/CD135 Antibody (A88079) at 1:400 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Enhanced Kit (RM00021). Exposure time: 60s.
Immunohistochemistry - Anti-Flt3/CD135 Antibody (A88079) - Antibodies.comEnlarge Image

Immunohistochemistry - Anti-Flt3/CD135 Antibody (A88079)

Immunohistochemistry analysis of paraffin-embedded rat pancreas using Anti-Flt3/CD135 Antibody (A88079) at a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
Immunohistochemistry - Anti-Flt3/CD135 Antibody (A88079) - Antibodies.comEnlarge Image

Immunohistochemistry - Anti-Flt3/CD135 Antibody (A88079)

Immunohistochemistry analysis of paraffin-embedded human liver cancer using Anti-Flt3/CD135 Antibody (A88079) at a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
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